Hypocreales » Ophiocordycipitaceae » Purpureocillium

Purpureocillium takamizusanense

Purpureocillium takamizusanense (Kobayasi) S. Ban, Azuma & Hiroki Sato, Int. J. Syst. Evol. Microbiol. 65: 2463 (2015)     

Index Fungorum number: IF 811842; Facesofungi number: FoF 13971

Parasitic on stinkbug. Sexual morph: Undetermined. Asexual morph: Synnemata 550–940 × 50–120 (x̄ = 730 × 87, n = 5) μm, gray, gregarious, branched, stipitate, apically powdery due to sporulation. Conidiophores penicillate, composed of septate, smooth-walled hyphae, terminating in group of phialides. Phialides 7–9.5 × 1.5–2.3 (x̄ = 7.8 × 2, n = 20) μm, hyaline, flask-shaped, smooth-walled, with a narrow neck, monophialidic. Conidia 2–3.5 × 1–2 (x̄ = 2.7 × 1.4, n = 25) μm, hyaline, broadly fusiform, aseptate.

Culture characteristics  Colonies on PDA reaching 5 cm after 30 days at 25 °C, gray-purple, circular, powdery, margin entire, reverse white gray.

Material examined  Thailand, Chiang Mai Province, Chiang Dao District, on stinkbug lying on ground, 12 August 2020, De-Ping Wei, CD0805 (MFLU 22-0271), living culture MFLUCC 22-0186.

Notes  Purpureocillium takamizusanense was originally found on a cicada in Japan and was described as an Isaria species by Kobayasi (1941). Ban et al. (2015) transferred this species to Purpureocillium and linked its teleomorph to Cordyceps ryogamimontana based on phylogenetic analyses. Purpureocillium takamizusanense growing on stinkbug has been documented on the website “Atlas of Invertebrate-Pathogenic fungi of Thailand”. In this study, we discovered P. takamizusanense from a stinkbug in northern Thailand and it phylogenetically clusters with P. takamizusanense (BCC49261) with high support. Morphologically, our isolate resembles P. takamizusanense in producing numerous, lilac-colored synnemata, bottle-shaped phialides and catenulate, fusiform to ellipsoidal conidia. Hence, we determined our isolate as a new collection of this species.

 

 

 

Purpureocillium takamizusanense (MFLU 22-0271). a Synnemata growing on hosts. b, c Enlargement of synnemata. d Synnema bearing conidiophores. e, f Conidiophores and phialides. g–j Conidia. Scale bars: c, d = 100µm, e, f = 20 µm, g–j = 3 µm (e–j stained with Congo red solution).

Reference:

 

Wei DP, Gentekaki E, Wanasinghe DN, Tang SM, Hyde KD. Diversity, molecular dating and ancestral characters state reconstruction of entomopathogenic fungi. Mycosphere. 13(2): 281351.

 

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Supported by 

Thailand Science Research and Innovation (TSRI)

project entitled:

"The future of specialist fungi in a changing climate: baseline data for generalist and specialist fungi associated with ants Rhododendron species and Dracaena species"

(Grant No. DBG6080013)

 

 

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