Paraisaria rosea
Paraisaria rosea D.P. Wei & K.D. Hyde, in Wei, et al., Frontiers in Microbiology 11(no. 608991): 11 (2021)
MycoBank number: MB834001, Facesoffungi number: FoF 07241
Parasitic on a larva of Coleoptera. Host buried in the soil, with the stroma erumpent from the ground. Sexual morph: Stroma up to 14.5 mm long, laterally emerging from the middle part of the larva body, simple, erect. Fertile head 4.5 × 4 mm, subglobose, pale pink at top and paler toward the base when fresh, pale yellow-brown when dry. Stipe 10 × 1.5 mm, white, straight, unbranched, glossy, cylindrical, inside not hollow. Perithecia 500–900 × 150–350 (x̄ = 762 × 256, n = 30) μm, completely immersed, ampulliform, ostiolate. Peridium 9–15 (x̄ = 12, n = 30) μm wide, composed of hyaline, thickwalled cells of textura angularis to textura globulosa to textura prismatica. Asci 230–390 × 3.5–6 (x̄ = 280 × 5, n = 15) μm, hyaline, cylindrical, unitunicate, eight-spored, possessing a prominent apical cap. Apical caps 5–7 × 2–6 (x̄ = 6 × 4, n = 20) μm, with a conspicuous tunnel throughout the center. Ascospores filiform, hyaline, breaking into secondary ascospores when mature. Secondary ascospores 4–11 × 1.5–2.5 (x̄ = 7.5 × 2, n = 30) μm, hyaline, cylindrical with truncate ends, smooth-walled, aseptate. Asexual morph: Hyphomycetous. Synnemata producing from the center of culture after 16 months incubation in dark environment, composed of loose, septate hyphae, white, filamentous, aerial, straight, branched, fasciculate, bearing shining droplets and conidiophores. Mycelium 2.4–3.7 (x̄ = 3, n = 10) μm in wide, septate, hyaline, smooth-walled. Conidiophores 33–48 (x̄ = 41, n = 10) μm in height, irregularly differentiate from the synnemata, sparse, gregarious, branched. Phialides 5.8–11.5 × 3–5.5 (x̄ = 8.6 × 4, n = 30) μm, ampulliform, 1-necked, hyaline, aseptate, enteroblastic, phialidic, monophialidic. Conidia 8–12 × 2–2.6 (x̄ = 9.8 × 2.3, n = 50) μm, hyaline, cylindrical, smooth-walled, aseptate, with round ends (Wei et al. 2021)
Culture characteristics: Culture was made from mycelium inside body of the host larva, slowly growing on PDA, reaching 1.3 cm in diam after incubated at room temperature (25 °C) for 50 days, convex, dense, with undulate edges, smooth surface become filamentous after forming aerial synnemata. The shooting conidia land on the surrounding culture and develop new colonies (Wei et al. 2021).
Known hosts and distributions: on larva of Coleoptera sp. buried in soil (China: Yunnan) (Wei et al. 2021).
Sequence data: KUMCC 20-0001 (SSU = MK752791, LSU = MK752849, ITS = MK752683, TEF = MK926450, RPB1 = MK882622) (Wei et al. 2021).
Sexual morph of Paraisaria rosea (Material examined: China, Yunnan Province, Kunming, Western hill Park on larva of Coleoptera sp. buried in soil, 27 July 2018, Deping Wei, XS2712 (HKAS 102546 – Holotype). (a) Stroma emerging from host. (b) Fertile head. (c) Transverse section of the fertile head. (d–f) Perithecia. (g) Peridium. (h,k,m) Asci. (i,j) Asci cap. (l) Part of ascus. (n–p) Secondary ascospores. Scale bars: (d) 1000 mm, (e) 300 mm, (f) 200 mm, (g,m) 30 mm, (h,k) 50 mm, (l) 20 mm, (i,j, n–p) 5 mm. (k mounted in Melzer’s reagent)) (Wei et al. 2021).
Asexual morph of Paraisaria rosea (KUMCC 20-0001, ex-type). (a,d) Upper and lower views of cultures on PDA after 50 days. (b,e) Upper and lower views of cultures on PDA after 16 months incubation in dark environments. (c,f) Enlargement of aerial synnemata produced on culture. (g) Synnema bearing conidiophores. (h–l) Phialides. (m) Conidia. (n,o) Irregularly aggregated conidia. Scale bars: (g) 100 mm, (h–l) 30 mm, (m–o) 5 mm. (h–k,m mounted in cotton blue reagent)) (Wei et al. 2021).
Phylogram generated from maximum likelihood analysis based on combined LSU, SSU, TEF1-a, RPB1, RPB2, and ITS sequence data. Bootstrap values for BI equal to or higher than 95%, ML and MP equal to or greater than 60% are placed on the notes. The newly generated sequences are indicated in blue bold. The host order of Paraisaria species and the generic names are labeled in the right side (Wei et al. 2021).