Cordyceps neopruinosa Mongkols., Noisrip., Khons. & Luangsa-ard, Mycol. Progr. 19(9): 976 (2020)
Index Fungorum number: IF 835366; Facesoffungi number: FoF 13962
Parasitic on insect. Sexual morph: Stromata 3 cm high, cylindrical, orange, multiple, branched, fleshy, stipitate. Fertile part 4 mm in length, 0.7 mm in wide, occurring in the middle and upper part of stroma. Perithecia 230–300 × 84–143 (x̄ = 273 × 115, n = 10) μm, ovoid, superficial. Peridium 15–35 (x̄ = 24, n = 20) μm, composed of thin-walled, orange cells of textura angularis. Asci 100–160 × 2–3.5 (x̄ = 129 × 3, n = 20) μm, filiform, with thickened apical cap. Ascospores immature.
Culture characteristics – Colonies on PDA reaching 3.5 cm after 45 days at 20 °C, orange-yellow, circular, margin entire, reverse yellow, with erected cylindrical, yellow synemata in circular arrangement near to the margin of colony.
Material examined – China, Yunnan Province, Kunming City, Xishan District, Western hill Park, probably on pupa of insect lying on leaf litter, 20 November 2021, De-Ping Wei, XS1101 (HKAS 102453), living culture KUNCC 21-10507.
Notes – Our specimen (HKAS 102453) was found on leaf litter in Yunnan Province, China. Regrettably, its host was not found with the fruiting bodies in the field and this specimen was not matured yet. We have obtained the pure culture by cultivating the tissue. The new collection groups with Cordyceps neopruinosa with significant support (Fig. 1). Cordyceps neopruinosa was introduced from a pupa of Limacodidae (Lepidoptera) in Thailand. The colonies growing on PDA are yellow, floccose, giving rise to awl-shaped synnemata, verticillate conidiophores and mariannaea-like conidial arrangement (Mongkolsamrit et al. 2020b). Our specimen has similar culture characteristics with those of C. neopruinosa.
Cordyceps neopruinosa (HKAS 102453) k Stromata.l Close-up of stroma. m Vertical section of perithecia. n Peridium. o Asci (o stained with Congo red solution).
Wei DP, Gentekaki E, Wanasinghe DN, Karunarathna SC, Tang SM, Hyde KD 2022 – Diversity, molecular dating and ancestral characters state reconstruction of entomopathogenic fungi in Hypocreales. Mycosphere 13: 281–351.